Expression of IFN-λ1 from Congjiang pigs and its effect on anti-PRRSV proliferation
نویسندگان
چکیده
An eukaryotic expression system of Congjiang pigs IFN-λ1 was constructed to obtain its in CHO-K1 cells and the inhibition effect pig on PRRSV proliferation verified. The plasmid pEGFP-PoIFN-λ1 from gene fragment transfected into cells. Expression detected by fluorescence microscopy Western blotting. influence assessed. results study showed that recombinant correctly. After transfection, green fluorescent signal microscopy. blot analysis revealed at different time periods after porcine expressed, with highest observed 36 h transfection. antiviral activity supernatant transfection determined micro cytopathic method, biological 2.1×103 U/mL. Quantitative PCR used detect PRRSV, significantly inhibited mRNA viral a dose- time-dependent manner. This established system, quantitative method it has significant inhibitory which lays foundation for future production drugs clinical application.
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ژورنال
عنوان ژورنال: Polish Journal of Veterinary Sciences
سال: 2023
ISSN: ['2300-2557', '1505-1773']
DOI: https://doi.org/10.24425/pjvs.2020.134687